CV GuillaumeLamour .pdf



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Mots-clés: surface cell biophysic biomaterial adhesion differentiation neuron nerve protein contact angle optic culture science biology physics

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Guillaume Lamour
Contact
Information

01/18/2010

Neuro-Physique Cellulaire
Universit´e Paris Descartes
UFR Biom´edicale
45, rue des Saints-P`eres
75006 Paris, France

Voice: +33-(0)1.42.86.21.08
Fax : +33-(0)1.42.86.20.85
Mobile: +33-(0)6.08.06.12.88
E-mails: guillaume.lamour@parisdescartes.fr
lamour99@hotmail.com

Research
Interests

Surface Physics applied to biological systems, cell motility related to the adhesive properties of
substrates, nanoscale surface organization of cell culture substrates, growth of self-assembled
monolayers (SAMs), wettability of solid surfaces, coating of biopolymers, protein adsorption

Education

Universit´e Paris Diderot, Paris, France
Ph.D Student in Biological Physics

Nov. 2006 - present

Advisor: Dr. Ahmed Hamraoui
Thesis Topic: “Influence of substratum energetic structuration on PC12 neuronal-cell
adhesion and differentiation.”
Doctoral School: Interdisciplinary graduate school for the science (IVIV ED387)
M. Sc., Cellular and molecular Biophysics (2006)
B. Sc., Physical-Chemistry (2004)
Research

Nov. 2006 - present

Ph.D Student

Working under the supervision of Dr. Ahmed Hamraoui, at the Biomedical Research Section
(UFR Biom´edicale), University of Paris Descartes, France.
– Cell culture of PC12 cells (neuronal model line) on modified substrates.
Results reported in Colloids and Surfaces B: Biointerfaces. Work on nanoscale surface
free-energy gradients effects on PC12 cells recently accepted in Biomaterials.
– Surface studies on substrates and cells by atomic force microscopy (AFM).
– Adhesive strengths between cells and substrates evaluated by interferometry.
– Antibody labelling of cytoskeleton-linked proteins and fluorescence observations.
– Extensive studies on growth of self-assembled monolayers of alkylsiloxanes on glass/silica
surfaces, with modulation of the nanoscale ordering of surface-exposed terminal groups.
– Extensive wetting studies by static and dynamic contact angle measurements:
Access to the surface free-energy distribution of self-assembled monolayers.
May - Aug. 2008

Visiting Research Scholar

Working under the supervision of Professor Eric Borguet at the Chemistry Department, Temple
University, Philadelphia, PA 19122, USA.
– Optical studies on self-assembled monolayers by sum-frequency generation (SFG), Fourier
transform infrared spectroscopy (FT-IR), AFM and contact angle measurements.
– Development of a simplified experimental apparatus for contact angle measurements, with
results submitted to the Journal of Chemical Education.
– Studies on fibrinogen adsorption on Poly-l-Lysine.

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Guillaume Lamour

Teaching

Sep. 2007 - Jan. 2008

Tutorial Lecturer

Physics classes (66h) at the College of Medicine, Universit´e Paris Descartes.
Level: 1st year undergraduate, with highly selective contest (15-25% of students admitted for
2nd year undergraduate courses)
– Diffusion: fluid statics, perfect fluid dynamics, real fluid dynamics;
Applications: hemodynamic, transports, transmembrane transports.
– Electricity: electric forces, potential and electrostatic fields, electric dipoles, conductors
in electrostatic equilibrium, conductive mediums and transient regimes;
Applications: modelization of a nervous fiber, electrically charged membranes.
– Optics: Descartes’ laws, Snell’s law, plane and spherical diopters;
Applications: thin lenses, birefringent lenses.

Publications

[1] Lamour G., A. Eftekhari-Bafrooei, E. Borguet, S. Sou`es, and A. Hamraoui. “Neuronal
adhesion and differentiation driven by nanoscale surface free-energy gradients,” (accepted
in Biomaterials, In Press)
[2] Lamour G., N. Journiac, S. Sou`es, S. Bonneau, P. Nassoy, and A. Hamraoui. “Influence
of surface energy distribution on neuritogenesis,” Colloids Surf. B: Biointerfaces 72 (2009)
208-218.
[3] Lamour G., A. Hamraoui, A. Buvailo, Y. Xing, S. Keulayan, V. Prakash, A. EftekhariBafrooei, and E. Borguet. “Contact angle measurements using a simplified experimental
set-up,” (submitted to the Journal of Chemical Education)
[4] Lamour G., and A. Hamraoui. “PC12 cell sensing of substratum surface energy landscape
through the activity of filopodia and lamellipodia,” (in preparation)

Meeting
Presentations

Physics of Cells: From the Edge to the Heart (embo)
Primo˜sten, Croatia
Guillaume Lamour and Ahmed Hamraoui.
Neuritogenesis induced by nanoscale surface energy gradients. Poster.

Sep. 2009

Forum of European Neurosciences Societies
Jul. 2008
Geneva, Switzerland
Guillaume Lamour and Ahmed Hamraoui.
Influence of substratum physical and chemical cues on neuronal differentiation and on propagation of the growing neurites. Poster.
European Biophysics Congress London
Jul. 2007
Imperial College, London, England
Guillaume Lamour and Ahmed Hamraoui.
Differentiation of PC12 neuronal cells on chemically modified surfaces in nerve-growth-factor
free medium. Poster.

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Guillaume Lamour

Professional
Skills

Cell Culture:
– Culture of PC12 cells.
– Drug treatment of cells (experience of cytochalasin).
– Installation and maintenance of cell culture devices.
Microscopy:
– Phase contrast, fluorescence, confocal.
– Atomic force microscopy (AFM). Contact & tapping modes. Ambient and fluid.
– Reflection Interference Contrast Microscopy (RICM).
Surface Modifications:
– Growth of self-assembled monolayers of alkylsiloxanes on bare glass.
– Coating of biopolymers (Poly-l-Lysine and Poly-l-Ornithine) on bare glass and plastic tissue.
– Installation and maintenance of chemical lab equipements.
Surface Analysis:
– Contact angle measurements, static and dynamic.
– Fourier transform infrared spectroscopy (FT-IR).
– Experience in vibrationnal sum-frequency generation (SFG).
Miscellaneous:
– Experience in protein purification and concentration methods.
– Experience in Enzyme-Linked Immunosorbant Assay (ELISA) technique.
– Hardware assembly and maintenance of the contact angle experimental apparatus.

Computer
Skills

– Office automation: Microsoft Office, LATEX
– Image managing: Adobe Photoshop & Illustrator, ImageJ, Irfanview
– Graphic processing: Igor, Origin
– Bibliographic managing: Web of Science, Pubmed, Endnote, Refworks
– AFM softwares: Veeco Nanoscope, Picoscan, WsXM
– Math software: Maple

Languages

– English: fluent (CLES certification: B2, 2008
GRE: quantitative: 700/800, verbal:520/800, analytical: 3/6), 2006
TOEFL: 77/120, 2006 )
– French: native speaker.
– Spanish: spoken.

Date:

Signature:

01/18/2010

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