Mondo K et al 2012.pdf

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Mar. Drugs 2012, 10


Figure 2. LC/MS/MS identification and verification of BMAA in a single great
hammerhead shark fin from South Florida Bay waters. (A) Triple quadrupole LC/MS/MS
verification of BMAA standard. The chromatographic spectra of the three major ions
produced from collision-induced dissociations of m/z 459 are: (top panel) protonated AQC
derivative fragment (m/z 171), the quantitation ion; (center panel) protonated-BMAA AQC
fragment (m/z 289), the first qualifier ion and (lower panel) protonated-BMAA fragment
(m/z 119), the second qualifier ion; (B) Representative triple quadrupole LC/MS/MS
verification of BMAA in a great hammerhead shark. Spectra are the same as in Column A.

We detected and quantified BMAA in the fins of all shark species with concentrations ranging
from 144 to 1836 ng/mg wet weight (Table 2). BMAA was not detected in six out of the total number
(n = 29) of individual fin clip specimens assayed. The results demonstrate high concentrations of
BMAA in shark fins collected in areas with or without active cyanobacteria blooms. We observed
considerable variability within the same shark species having a similar body length and taken from the
same collection sites. For example, the bonnethead shark had BMAA concentrations that ranged from
320 to 1836 ng/mg over a range of only 76 to 79 cm. Of the 7 members of the elasmobranch family
surveyed, both the nurse shark and the blacktip shark had fin clip samples where BMAA was not
detected (Table 2). Interestingly, the two samples taken from nurse sharks sampled in Florida Bay
were positive for BMAA while only one of the five sampled from Biscayne Bay had a quantifiable
peak (Table 2). There was no apparent correlation of BMAA concentration with the size of the shark
or lifespan at sampling.