InertSustainSwiftC18 Catalog ITM.pdf


Aperçu du fichier PDF inertsustainswiftc18-catalog-itm.pdf - page 4/22

Page 1 2 3 45622



Aperçu texte


InertSustainSwift C18 HPLC Columns

Rapid Analysis of Dissolution Test of Formulations
InertSustainSwift C18 is optimally bonded offering moderate retentivity of analytes without accumulating strong
hydrophobic contaminants in the column. Most importantly, the optimized surface area (200 m2/g) and carbon load
(9.0 %) to the silica gel enables rapid elution of samples in isocratic methods which creates the column to be highly
efficient for dissolution test of formulations.
Conditions
Column Size
Eluent

1

: 5 μm, 50 x 4.6 mm I.D.
: CH3CN/H2O/CH3COOH
= 500/500/1, v/v/v
: 1.0 mL/min
: 30 ℃
: 100 μL
: UV 225 nm
: 1. Valsartan

Flow Rate
Col. Temp.
Inj.Vol.
Detection
Sample

InertSustainSwift C18

1

The adsorption of peak leads
to poor peak shape with longer analysis time.

Other Brands C18 Column

Rapid Column Equilibration
When columns are not equilibrated enough in gradient methods, irreproducible retention time of analytes, baseline
drift and lower sensitivity in LC-MS and LC-MS/MS methods can be experienced. When discussing about rapid analysis
in gradient methods, reducing the column equilibration time is significantly important to inject the next sample to
achieve total rapid analysis. As shown below, InertSustainSwift C18 provide rapid column equilibration.

2

1

2

1

Equilibration
for 5 min
0

2

4

6

8

10

Time (min)

0

2

Baseline
Drift

4

6

8

10

Time (min)

Equilibration
for 10 min
0

2

4

6

8

10

Time (min)

Full Column
Equilibration

0

2

4

0

2

4

6

8

10

6

8

10

Organic Solvent (%)

Gradient Time Program
100

Equilibration Time
5 minutes
10 minutes
15 minutes
20 minutes

5
0

8

13

Time (min)

6

8

10

Time (min)

InertSustainSwift C18

0

2

4

18

Time (min)

XBridge C18

1. Methamidophos

2. Acephate

Time (min)