Stagorn Corals Transplantation .pdf



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Transplantation of Staghorn Corals
Using Injured Coral Tissue Recovery to
Increase Apical Polyp (AP) Generation
& Overall Productivity
Author : Cédric Guignard
Email : Cedric@seamarc.com
Co-Author : Thomas Le Berre
Email : Thomas@seamarc.com
Seamarc Pvt Ltd
Republic of Maldives

Breakage Injuries (Cut)
Acropora muricata

Cut recovery after 5 months
Acropora muricata

Experiment 1:Basal & Proximal Fragments
AP Generation (APG)
Acropora muricata
2 batches of 40 fragments
3 months observation
100% survival
Indicator: Quantity of AP

Experiment 1: Results
Basal transplants

T-test

Median

6 AP

96.4 % CI

5 to 7 AP

Bt = Pt
Proximal transplants
Median

3 AP

95.9 % CI

2 to 3 AP

Conclusion 1
Basal fragments APG
>
Proximal fragments APG

Questions
Is recovery tissue responsible for generating
AP ?

How to verify?
By inflicting artificial injuries to test branching
inducement

Experiment 2 : Branching
inducement by artificial injuries

Artifcial injuries

Acropora aspera
40 fragments
97.5% survival
0.45 EAP / XCut

Acropora pulchra
40 fragments
97.5% survival
0.25 EAP / XCut

Extra Cut (XCut)

Day 0

Day 21

Day 49

Conclusion 2

Recovery tissue is responsible for generation
of AP

Questions
Can we use it to increase fragment productivity?

Next step
Apply the XCut technique to Acropora
muricata
Standard shape and size fragments
Need a simple productivity indicator

Experiment 3 : XCUT and AP removal
Reference fragment

 Acropora muricata
 Proximal fragments
 2 batches of 32 fragments

Xcut fragment

 XCut and AP removal
 10 week observations
 100% survival

Productivity Indicator
Radial Polyp Crown (RPC)
No RPC
2 RPC

5 RPC

Day 0

Day 14

Day 35

Day 49

Day 70

Results
Branching

Productivity
RPC Productivity

Mean Apical Polyps generated
8

70

7

60

6
5

40
RPC

AP quantities

50

4

30
3

20

2

10

1
0

0

Ref

1

Xcut

T-test not significant

Ref

1

Xcut

T-test significant

Conclusion 3
The technique did not induce any significant increase of
the total APG but enabled a significant increase of the
total productivity (+52%).

Artificial injuries induced a significant increase of both
APG and productivity between transplant extremities,
without affecting survival rate.

Experiment 4 : Fragmentation Rate &
Productivity
48 fragments of 3 cm


Acropora muricata



Basal fragments



50 days observation



100% survival



Total length = 144 cm

24 fragments of 6 cm

16 fragments of 9 cm

366 AP
1951 RPC
297 AP
1359 RPC

195 Tips
1116 RPC

Analysis
The fragmentation rate induced a significant increase of
both APG and Productivity
2500

APG
2000

RPC

1500

1000

500

0

Frag 16

Frag 24

Frag 48

Positive correlation (Pearson ; alpha= 0,05) between
fragmentation rate and productivity > 0.999

Conclusion 4
• Fragmentation is highly beneficial for
the transplantation of Acropora muricata,
through the increase of recovery tissues
areas

This technique enabled a productivity
increase of 75% between the batches of 9
cm and 3 cm fragments with the same
initial stock

General Conclusion
To optimise the use of Acropora muricata mother colony during reef
propagation project:
 Use preferentially basal fragments for higher branching rate
 Remove Apical Polyps of proximal fragments
 Fragment it in many small fragments of at least 3 cm
 Inflict artificial injuries to increase productivity and
improve fragment shape

Perspectives
 Test the technique on other staghorn species
 Increase the fragmentation rate to assess its optimum
 Use skeleton weight as calcification indicator

Special Thanks
Four seasons Resorts Maldives
Magali Marion
Sebastien Monier



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