PwptAB .pdf



Nom original: PwptAB.pdfTitre: Interactions between Beta-amyloid and supported lipids bilayer by Fluorescence Correlation SpectroscopyAuteur: vdubois

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Department of Chemistry

Interactions between Beta-amyloid and
supported lipids bilayer by Fluorescence
Correlation Spectroscopy

6/15/2015

Page 1

Department of Chemistry

Table of Contents


Introduction



Supported lipids bilayer formation



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6/15/2015

Title of the presentation, Author

Page 2

Department of Chemistry

Introduction
Amyloid plaque is an important symptom of
Alzheimer’s desease.
Plaques are composed of aggregated
β-Amyloid (38 to 43 a.a. peptide) from APP.
Both APP and β-Amyloid interacts with
neuron’s membrane.
 What are the direct, non-biological, effects
of β-Amyloid interactions with lipids? How are
these interactions governed?

6/15/2015

Title of the presentation, Author

Page 3

Department of Chemistry

To investigate the interaction with membrane,
a mimick of lipids bilayer is used as substitute
Vesicles are adsorbed on a coverslip and
assembled to form the « fake-membrane »
Supercritical Angle Fluorescence and Fluorescence Correlation Spectroscopy
are used to characterize the changes
in lipid’s behaviour.

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Page 4

Department of Chemistry

Supported lipids bilayer formation
• 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)
• 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS)
• 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
(DOPE tagged with Atto647N)

D = 2.5 – 3.5 µm²/s

6/15/2015

Title of the presentation, Author

Page 5

Department of Chemistry

Supported lipids bilayer formation
• 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)
• 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS)
• 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine7
(DOPE tagged with Atto647N)
• Cholesterol
D = 1.47 µm²/s
Anomalous diffusion due to Cholesterol

6/15/2015

Title of the presentation, Author

Page 6


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