First commercial serological tests for Zika virus .pdf

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First commercial serological tests for Zika virus
ELISA and indirect immunofluorescence tests (IIFT) have
been developed for sensitive and specific detection of
antibodies against Zika virus in patient serum samples. The
assays are suitable for diagnosing acute infections as well as
for disease surveillance. In particular, serological analyses can
aid the differentiation of infections with Zika virus, dengue
virus and chikungunya virus, which manifest with similar
symptoms and are endemic in much the same geographic
regions. Anti-Zika Virus ELISA (IgM or IgG) are based on
highly specific recombinant Zika NS1 protein which avoids
cross reactivity with other flaviviruses. Data from panels of
well characterized sera have confirmed that there is no cross
reactivity with flaviviruses including dengue, West Nile, yellow
fever and Japanese encephalitis viruses. In studies on
clinically and serologically characterized samples the IgM and
IgG ELISA showed 100% sensitivity and 100% specificity.
Anti-Zika Virus IIFT (IgM or IgG) utilize Zika virus-infected
cells as the antigenic substrate. Positive and negative results
are evaluated by fluorescence microscopy. With the Arboviral
Fever Mosaic 2 the Zika virus substrate is incubated in parallel
with substrates for chikungunya virus and dengue virus
serotypes 1 to 4.
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