Tresset, Postdoc Position, 2017 .pdf
Nom original: Tresset, Postdoc Position, 2017.pdfAuteur: TRESSET Guillaume
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Laboratoire de Physique des Solides
rue Nicolas Appert, Bâtiment 510
91405 Orsay Cedex, France
Tel: +33 (0)1 69 15 53 60
Fax: +33 (0)1 69 15 60 86
Postdoctoral position: High spatiotemporal resolution dynamics
of genome packaging in viral nanoshells
An 18month postdoctoral position is open to work on the physics of virus selfassembly. This
is a joint project between the Laboratory for SolidState Physics (Guillaume Tresset) and the
Institute for Integrative Biology of the Cell (Stéphane Bressanelli) at the Université
ParisSaclay in France. The successful candidate will be either a soft matter physicist with
interest in biological systems or a biochemist with interest in physics approaches. He/she will
work with viral capsid proteins, RNA and synthetic polyelectrolytes. An experience with
scattering techniques (SAXS/SANS) will be favorably considered.
Singlestranded (ss)RNA viruses are major public health issues (e.g. hepatitis C, Dengue, and
Zika viruses) as well as serious economic and veterinary concerns worldwide. ssRNA viruses
have in common a simple protein nanoshell – the capsid – surrounding and protecting the
RNA genome. Some simple ssRNA viruses can selfassemble in vitro forming infectious
particles from purified components. There are currently no physical models accounting
reliably for the dynamic pathways along which the hundreds of molecular building blocks
making up a virus fit into the final structure with a pinpoint accuracy.
This project aims at elucidating the dynamic pathways of genome packaging into icosahedral
viral nanoshells. The latter will be derived from plant viruses that have been used for physical
 and nanotechnological investigations. RNA with different lengths as well as synthetic
polyelectrolytes with varying charge density and topology will be packaged in vitro through a
selfassembly process. The dynamics will be probed with cuttingedge ensembleaveraging
techniques including timeresolved smallangle Xray scattering (TRSAXS) with high
brilliance synchrotron source supplemented by custommade algorithms for data processing
[2,3]. Smallangle neutron scattering (SANS) and cryotransmission electron microscopy
(cryoTEM) will provide complementary information at equilibrium. We expect to catch the
transition states occurring over a few tens of milliseconds and to reconstruct the structure of
intermediate species with nanometer resolution.
 G. Tresset et al., Weighing polyelectrolytes packaged in viruslike particles, Phys. Rev. Lett. 113
 D. LawHine et al., Reconstruction of the disassembly pathway of an icosahedral viral capsid and
shape determination of two successive intermediates, J. Phys. Chem. Lett. 6 (2015) 34713476
 G. Tresset et al., Norovirus capsid proteins selfassemble through biphasic kinetics via longlived
stavelike intermediates, J. Am. Chem. Soc. 135 (2013) 1537315381
Contact: Guillaume Tresset
Duration: 18 months
Monthly gross salary: starting from €2500