2017 08 ad for biochemist position f .pdf

Nom original: 2017-08 ad for biochemist position_f.pdfTitre: 2017-08 ad for biochemist position_fAuteur: yoyo

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Post-doc / engineer position in biochemistry
Prokaryotic Cell Development Lab
INRA of Jouy-en-Josas, France
We study emerging questions in the fields of bacterial cell morphogenesis and the bacterial
cytoskeleton using a combination of high-resolution fluorescence microscopy with powerful
genetic, genomic, biochemical, biophysical and systems biology approaches
We are located in the Microbiology Department of the INRA of Jouy-en-Josas, in the Paris
area (20 km south-west of Paris and 7 km east of Versailles).
A position for a post-doc or engineer is currently open in our group for a skilled biochemist.
The fellow will be part of a multidisciplinary and collaborative project in the framework of a
European ERC Starting grant. The project aims at deciphering the properties and mode of
action of bacterial proteins from the actin family using a combination of genetics, cell biology
and biochemistry approaches. The hired fellow will team up with geneticists/microscopists
and will take in charge the biochemistry part of the project.
The hired biochemist will be in charge of purifying and performing a full in vitro
characterization of bacterial proteins from the actin family (notoriously difficult to work
with), including their polymerization and nucleotide degradation properties. A mix of
standard (colorimetric assay, light scattering or SPR, TEM…) and advanced (fluorescent
tagging for live in vitro assay on TIRF microscopy, AFM…) approaches will be used, in order
to set up a standardized procedure for phenotypic characterization. Then, mutants isolated by
other member of the project will be characterized following this procedure.
· The candidate should hold a PhD, but experienced and autonomous skilled biochemists with
a Master degree would also be considered.
· Strong theoretical and practical experience in biochemistry (demonstrated with a good
publication record) is mandatory: the fellow will have purified and characterized proteins,
using at least standard approaches (affinity and exclusion size chromatography, LS, SPR…).
· Experience with fluorescent labelling and advanced microscopy techniques (TIRF, AFM…)
applied to in vitro protein studies will be an important asset.
· Experience with proteins having polymerization properties, membrane affinity and/or
ATPase activity are a plus.
· Ability to work in a team.
· This ERC-funded position is available immediately for a period of 12-18 months depending
on the starting date, which is flexible until the end of 2017. The project ends in January 2019.
Contact: Arnaud Chastanet arnaud.chastanet@inra.fr

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