2018 May Steph CV .pdf



Nom original: 2018 May_Steph CV.pdf
Titre: 2018 May_Steph CV
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HEIL Stéphanie
Mobile: +33633880214/0708876529
Email: stephanie.da.silva@liu.se

Blåvik Ljusnaberget 1
590 14 Boxholm, Sweden

EDUCATION.
PhD
October 2010- October 2013
PhD in Enzyme and bacterial engineering
Institut National des Sciences Appliquées (INSA) at CNRS (Biosystems and Process Engineering
Laboratory (LISBP, INSA)) and INRA (Neurogastroenterology and Nutrition), Toulouse (France).
“Consequences of a chronic stress on intestinal mucus barrier: effect of the probiotic strain
Lactobacillus farciminis”. DOI: 10.13140/RG.2.1.1948.9529
Supervised by Dr. Muriel Mercier-Bonin, collaboration with Pr. Vassilia Théodorou and Dr.
Etienne Dague.
Aim. Evaluation if a chronic stress modified the number of goblet cells, Muc2 expression and Oglycosylation and if a LF treatment prevented these alterations and at detection of the spatial
localization of the exogenously applied probiotic Lactobacillus farciminis in rats, after exposure to
IBS-like chronic stress.
Methods. IBS Animal models, histology, Mass Spectrometry, Microscopy (Fluorescence, AFM, SEM
and TEM), FISH, qPCR, intestinal paracellular permeability, and visceral sensitivity
Animal Welfare Level 1
February 2009
CNRS, Marseille, France. Updated with Swedish Legislation (Module A) in 2014. Validated
equivalence to FELASA C.
Bachelor and Master Degrees
September 2008 – September 2010
Bioengineering, specialization in Molecular and Cellular Biotechnologies
University Paul Sabatier, Toulouse III, France (Institut Universitaire Professionnalisé (IUP))
DEUG Degree (2-years University Degree)
Cellular biology and Animal physiology
University Paul Sabatier, Toulouse III, France
Preschool for Veterinary College
BCPST section
High School Ozenne, Toulouse, France
Baccalauréat Scientifique
High School diploma, scientific option, equivalent to A-Levels
High School L. Limosin, Limoges, France

September 2006 – June 2007

September 2005 – June 2006

June 2005

EMPLOYMENT AND SKILLS.
Principal research Engineer
June 2018 – now
Linköping University, Sweden (IKE, Surgery)
Epithelial regeneration in wound healing in mice
Methods. Development of acute mice-colitis models using chemicals
compounds (DSS) for studying Wnt signaling pathway in wildtype and genetically engineered mice. Creation by breeding of the
CCNY-Flox mice model and genotyping of the litters obtained by
PCR. Characterization of the response induced by DSS and the
involvement of CCNY in wound healing (immunofluorescence,
Western-Blot…). Co-supervision of Master trainee (AugustDecember 2017 (vectors generation, gene editing by CRISP-Cas9
system, cell culture and transfection, characterization of edited cell
line). Supervised by Dr. Stefan Koch.
OPA Energy AB
2018 – now
Co-founder, substitute board member, community manager and
administrative support.
Post-doctoral researcher
February 2017 – February 2018
Linköping University, Sweden (IKE, Surgery)
Epithelial regeneration in wound healing in mice
Methods. Development of acute mice-colitis models using chemicals
compounds (DSS) for studying Wnt signaling pathway in wildtype and genetically engineered mice. Creation by breeding of the
CCNY-Flox mice model and genotyping of the litters obtained by
PCR. Characterization of the response induced by DSS and the
involvement of CCNY in wound healing (immunofluorescence,
Western-Blot…). Co-supervision of Master trainee (AugustDecember 2017 (vectors generation, gene editing by CRISP-Cas9
system, cell culture and transfection, characterization of edited cell
line). Supervised by Dr. Stefan Koch.
Post-doctoral researcher
November 2013 – August 2017
Linköping University, Sweden (IKE, Surgery, November 2013November 2015; February 2016-February 2017)
Uppsala University, Sweden (November 2015-February 2016)
Barrier function in animal models of inflammatory bowel disease
Methods. Development of mice-colitis models (TNBS and DSS) for

the characterization of the anti-inflammatory response induces by a
new generation of PPAR-gamma agonists with soft-drugs
properties, in collaboration with Albireo AB and Lund University.
Characterization of the inflammatory response induced by DSS in
mice regarding specific targets as VIP. Comparison with tissues
from Ulcerative colitis or Crohn’s patients at different levels
(cytokines and inflammatory response, genes, macroscopic
evaluation...). ELISA, immunohistochemistry, fluorescence
microscopy, histology, IBD animal model, ethical application
writing, patient’s surgical tissues bank, RT-qPCR (collaboration
with Lund University). Congress presentation at UEGW in 2015.
Directed by Pr. Johan D. Söderholm.

Problem solver

Industrious

Creative

Self-motivated and
determined

Initiative taker

Ability to work well
under pressure

Organized and meet
deadlines

Ability to take on
responsibilities

Team player

Written and verbal
communication skills
(oral presentations
and articles)

I.T. skills including
experience in
Microsoft Office
(Word, Excel,
PowerPoint),
Photoshop and
ImageJ, Graph Pad
Prism5

Master Research project
January 2010 – August 2010
Pierre Fabre Dermo-cosmétique (Toulouse, France). Full-time
immersive project in Industry (Confidential signed NDA)
Characterization of the inflammatory response of a reconstructedepithelium model
Methods. RT-qPCR for Gene expression on tissues by RT-qPCR,
immunohistochemistry for protein localization and expression
level, immunohistochemistry, cellular biology (primary cells and
cell lines, cells viability, cell test for activation of NF-ҡB pathway
and cellular proliferation), protein quantification by Luminex.
Directed by Marguerite Levêque.
Master Research project
February 2009 – July 2009
Sanofi-Aventis (Toulouse, France). Full-time immersive project in
Industry (Confidential signed NDA)
Cloning, expression and activities of a chaperon protein in order to test
potential inhibitors by activity test in vitro for oncology and infectious
disease drug research
Methods. E. coli culture, gene cloning, expression and protein
purification, development of activity test for protein in vitro in
order to characterize drugs compounds. Directed by Dr. Sandra
Silve.
Veterinary assistant
Summer term 2007, 2008 and 2009
LAPVSO (Laboratory of veterinarian anatomy-pathology of the
South West, Toulouse, France)
Assistant to the Veterinarian, from the total piece of tissue to the
preparation for diagnosis. Role: read the records of the samples
send for analysis in order to guide the veterinarian for the
preparation of the samples and take note all the comments from the
veterinarian.

OTHER INFORMATIONS.
Forum Scientium member

2017 - now

ECCO (European Crohn´s and Colitis Organization) member

2017 - now

Facebook community manager
2018 – now
Management and announcement of upcoming activities for MIIC group at Linköping University.
MIIC SAP Seminar organization
February 2017 – December 2017
Each Monday from February 2017 for PhD student and post doc within MIIC group at Linköping
University. Booking rooms, contacting and planning speakers, sending planning and reminder.
Linköping Junior Faculty
Member, participation to Lunch Meeting, Workshop, seminars…

September 2014 - now

Involved in Reference Group meeting where decisions are taken and in Working group dedicated to
specific questions.

A2D2 (Association des anciens des Doctoriales)
Active member and Secretary of the Association.

2013 - 2014

This association allows the PhD students who participate to the annual event "Les Doctoriales" to
keep in touch and meet PhD graduated scientist in order to create an active and multidisciplinary
scientific network in the county of Toulouse. Diverse activities (Meetings, activities on week-end...)
are organized regularly. The board members also have to represent the association during the
"Doctoriales" annual event.
APro’Doc (Avenir professionnel des Doctorants)
2010- 2011
Member of the organizational Committee.
APro’Doc is a volunteer organization for PhD students is the county of Midi-Pyrenees in France.
This organization aimed to organize an annual event in order to develop contacts between
Academia and Industry. In 2011, we proposed lectures and workshops for 200 PhD students and
major companies as Pierre-Fabre, Total, Continental, Meteo-France, innovative start-up companies
(Genticel…) were presents to give advices during all the day to the students.
Center for the Protection of the Wildlife "SOS FAUNE SAUVAGE"
2002 - 2006
Volunteer animal keeper for protected animal species. Vice secretary of the association (2004-2006).
Center with State agreement (Verneuil sur Vienne (87, France)). The purpose is to collect the
wounded animals of the protected Wildlife and to release them in their natural environment.

LANGUAGES.
French: mother tongue, native speaker
English: fluent written and spoken
Spanish: basic communication skills
Swedish: basic communication skills

INTERESTS.
Dances (Salsa and Tango)
Handcraft (painting, drawing, jewelry…)
Outdoor activities (hiking, fishing, camping)
Reading

REFERENCES.
Pr. Johan D. Söderholm

Dr. Åsa V. Keita

Pr. Vassilia Théodorou

Dr. Muriel Mercier-Bonin

Linköping University
IKE, Division of Surgery
SE-581 85 Linköping
Sweden

Linköping University
IKE, Division of Surgery
SE-581 85 Linköping
Sweden

johan.d.soderholm@liu.se

asa.keita@liu.se

INRA UMR 1331 TOXALIM
Equipe 4 NGN
180, chemin de Tournefeuille
31027 TOULOUSE cedex 3
France
vassilia.theodorou@toulouse.inra.fr

INRA UMR 1331 TOXALIM Equipe 4
NGN
180, chemin de Tournefeuille
31027 TOULOUSE cedex 3
France
muriel.mercier-bonin@toulouse.inra.fr

APPENDIX
DETAILED SYNOPSIS OF PhD RESEARCH .
Background. Despite a large body of literature incriminating mucus alterations in the pathogenesis
of Intestinal Bowel Diseases (IBD), structural and physical changes in the mucus layer remain
poorly understood in the micro-inflammatory context of Irritable Bowel Syndrome (IBS).
Moreover, some probiotic treatments prevent stress-induced intestinal epithelial barrier
impairment but little is known about their influence on intestinal mucin structural modifications
and mucus properties induced by stress. Thereby, this study aimed at evaluating whether (i) a
chronic stress modified the number of gut goblet cells and Muc2 expression and O-glycosylation,
(ii) L. farciminis (LF) treatment prevented these alterations and (iii) observed effects were related to
the in vivo colonization capacity of LF.
Main results and conclusions. Water Avoidance Stress (WAS) did not modify neither the number of
intestinal goblet cells nor Muc2 expression. Mass Spectrometry analysis demonstrated that Oglycosylation of mucins was strongly affected by WAS, and confirmed in another model of IBS
(maternal deprivation model). Under stress conditions, the mucus layer, showed a flattened
morphology, probably indicative of a loss in its cohesive properties. The mucus layer alteration
was, thus, in relation with epithelial barrier impairment and visceral hypersensitivity. LF
administration prevented WAS-induced functional, biochemical and physical changes of mucus.
The presence of LF in the ileum and colon was confirmed and we observed that Segmented
Filamentous Bacteria (SFB) population was reduced by LF.
Chronic stress induced functional changes in rats, as well as a shift in mucin O-glycosylation rather
than changes in mucin expression, resulting in a loss of mucus layer cohesive properties. These
results confirm that LF is a valuable probiotic in the IBS management.
Methods. IBS Animal model (WAS and maternal deprivation), histological, Mass Spectrometry,
Microscopy (Fluorescence, AFM, SEM and TEM), bacterial localization by Fluorescence In Situ
Hybridization (FISH), qPCR, intestinal paracellular permeability, visceral sensitivity.

CONFERENCES, PRESENTATION AND COURSES ATTENDED .
Courses Attended
Bengt Ihre Research School
September 2016 – October 2017
Post doc program that aims to educate individuals who has completed their PhD in the
gastrointestinal field in leadership skills and writing of grant applications in translational
gastroenterological research; program opened for both clinical and basic science researchers and
provides a translational network of upcoming opinion leaders in Swedish gastroenterology.
INRA Summer School
INRA department AlimH (France), 2014 thematic “Nerve System and Nutrition”
One week program, lectures and presentations, articles comments and debates.

June 2014

Congress participations
Oral communications
- Congress “Biofilms for Biotechnologies and Bioenergy” (2012, Narbonne, France)
- 18th International Symposium of Lactic Bacteria Club (2012, CBL, Clermont-Ferrand,
France)
- 4th Health Food Symposium (2012, Toulouse, France)
- Digestive Disease Week (2013, Orlando, USA) and attribution of Travel grant

-

Annual meeting of the French Group in Neuro-Gastroenterology (2014, GFNG, Bordeaux,
France)
United European Gastroenterology Week (2015, UEGW, Barcelona, Spain) for Poster in the
Spotlight Session and attribution of Travel Grant

Main Presented Poster
- Lab10 (2011, Netherlands)
- Biofilms 5th (2012, Paris, France)
- Cell Symposia: Microbiome and Host Health (2013, Lisbon, Portugal)
- Meeting INRA-Rowett (2014, Aberdeen, Scotland)
- United European Gastroenterology Week (2015, UEGW, Barcelona, Spain)

AWARDS AND GRANTS.
Travel grants for DDW
2013
GFNG and SFN (French Societies) for Digestive Disease Week (DDW), Orlando, US (1 000 euros)
Travel grants for UEGW
UEGW Travel grant, Barcelona, Spain (1 000 euros)

2015

UEG Visiting Fellowship Programme for researchers
2018
Visiting Fellowship at the Hubrecht Institute for 2 weeks in 2019 (1250 euros) and free registration
to the subsequent UEG Week

PUBLICATIONS AND CONFERENCE PAPERS.
Stéphanie Da Silva, Alessandro Mancuso, Myriam Mercade-Loubière, Christel Cartier, Marion
Gillet, Afifa Ait-Belgnaoui, Pascal Loubière, Catherine Robbe-Masselot, Vassilia Theodorou,
Muriel Mercier-Bonin. Intestinal mucus alterations induced by chronic stress are linked to changes
in mucin O-glycan structure rather than MUC2 expression: prevention by a probiotic treatment,
May 2013, Gastroenterology Vol. 144, Issue 5, Supplement 1, page S-88.
Conference paper, published abstract of oral presentation at DDW, Orlando, 2013.
Stéphanie Da Silva, Catherine Robbe-Masselot, Afifa Ait-Belgnaoui, Alessandro Mancuso,
Myriam Mercade-Loubière, Christel Salvador-Cartier, Marion Gillet, Laurent Ferrier, Pascal
Loubière, Etienne Dague, Vassilia Theodorou, Muriel Mercier-Bonin. Stress disrupts intestinal
mucus barrier in rats via mucin O-glycosylation shift: prevention by a probiotic treatment. Am J
PhysiolGastrointest Liver Physiol. 2014 Aug 15;307(4):G420-9. doi: 10.1152/ajpgi.00290.2013.
Stéphanie Da Silva, Arthur Raymond, Myriam Mercade-Loubière, Christel Salvador-Cartier,
Renaud Léonard, Afifa Ait-Belgnaoui, Pascal Loubière, Catherine Robbe-Masselot, Vassilia
Théodorou, Muriel Mercier-Bonin. Spatial localization and binding of the probiotic Lactobacillus
farciminis to the rat intestinal mucosa: influence of chronic stress. Plos One. 2015 Sep
14;10(9):e0136048. doi: 10.1371/journal.pone.0136048. eCollection 2015.
Stéphanie Da Silva, Åsa V. Keita, Sofie Mohlin, Sven Påhlman, Ingrid Påhlman, Johan D.
Söderholm. A novel PPAR-gamma agonist, AS002, induces PPAR-gamma activity in ulcerative
colitis mucosa, and prevents and reverses inflammation in DSS colitis. United European
Gastroenterology Journal. October 2015; 3 (5 suppl), 23rd United European Gastroenterology Week
Barcelona, Spain, October 2015 Abstract Issue, page A590 (P1527).

Conference paper, published abstract of oral “Posters in the Spotlight” presentation UEGW,
Barcelona, 2015.
Marion Rincel, Amandine L. Lépinay, Yoottana Janthakhin, Gwenaëlle Soudain, Sophie Yvon,
Stéphanie Da Silva, Corinne Joffre, Agnès Aubert, Alexandra Séré, Sophie Layé, Vassilia
Theodorou, Guillaume Ferreira, Muriel Darnaudéry. Maternal high-fat diet and early-life stress
differentially modulate spine density and dendritic morphology in the medial prefrontal cortex of
juvenile and adult rats. Brain Struct Funct. 2017 Oct 11. doi: 10.1007/s00429-017-1526-8.
Carine Signoret, Elaine Ng, Stéphanie Da Silva, Ayco Tack, Ulrikke Voss, Helga H. Lidö, Cédric
Patthey, Madelene Ericsson, Jenny Hadrévi and Chanchal Balachandran. Well-being of earlycareer researchers in Sweden: insights from a national survey. Higher Education Policy, 2018 Feb.

doi: 10.1057/s41307-018-0080-1.

Stéphanie Da Silva, Åsa V. Keita, Sofie Mohlin, Sven Påhlman, Vassilia Theodorou, Ingrid
Påhlman, Jan P. Mattson, Johan D. Söderholm. A novel topical PPARγ agonist induces PPARγactivity in ulcerative colitis mucosa and prevents and reverses inflammation in induced-colitis
models. Inflamm Bowel Dis. 2018 Mar 19;24(4):792-805. doi: 10.1093/ibd/izx079.
Stéphanie D.S. HEIL, Maite Casado-Bedmar, Johan D. Söderholm and Åsa V. Keita. Increased
number of mast cells, vasoactive intestinal polypeptide and its receptor in patients with
Ulcerative colitis, ileal Crohn’s disease and in mice with colitis. Neurogastroenterology and
Motility, May 2018, In revision.



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