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Exploration de la dysfonction vasculaire en RC 2012 100%

central régional Local Une régulation locale complexe T = P.r ATTEINTES FONCTIONNELLES τ=µ µ.V/r Tonus Myogénique contrainte pariétale Muscle Lisse Matrice extracellulaire ATTEINTE STRUCTURELLE Cisaillement Neuro-humoral Endothélium SNV Hormones BaroReflexe Vasomotricité Débit-Dépendante Remodelage vasculaire Flow Pressure Nle ↓ dimensions ↑ dimensions ↓ lumen ↓ lumen no medial hypertrophy medial hypertrophy ∆ shear stress ∆ transmural pressure By endothelium By endothelium and smooth muscles Rôle clé de l’endothélium • • • • Contrôle du tonus vasculaire Inhibition de l’aggrégation plaquétaire Modulation de la migration lymphocytaire Modulation dela perméabilité vasculaire • Fonction endothéliale :

https://www.fichier-pdf.fr/2016/02/14/exploration-de-la-dysfonction-vasculaire-en-rc-2012/

14/02/2016 www.fichier-pdf.fr

Explorations vasculaires chez l animal 78%

abdelali.agouni@etud.univ-angers.fr Laboratoire de Biologie Neuro-Vasculaire Intégrée UMR CNRS 6214-INSERM U771 Faculté de Médecine, Angers La paroi vasculaire Valve Tunique intima (endothelium) Membrane élastique Tunique media Limitante élastique interne Tunique externe (adventice) Artère Veine La paroi vasculaire Endothélium Intima Limitante élastique interne Media Adventice Limitante élastique externe L’endothélium vasculaire est un organe couche monocellulaire la plus interne au vaisseau L’endothélium vasculaire est un organe 1 X 1012 cellules Masse:

https://www.fichier-pdf.fr/2016/02/14/explorations-vasculaires-chez-l-animal/

14/02/2016 www.fichier-pdf.fr

PIIS147149220200065X 76%

Babesia bovis-infected red blood cells (IRBC) can adhere to the capillary endothelium of blood vessels of various tissues, a behavior that could be abrogated by host antibodies recognizing parasite antigens on the IRBC surface.

https://www.fichier-pdf.fr/2018/03/05/piis147149220200065x/

05/03/2018 www.fichier-pdf.fr

Pathophysiologie nitrite 69%

Following these early events, these cytokines induce a number of cellular processes that culminate in the chemoattraction of neutrophils, adherence of neutrophils to cerebromicrovascular endothelium, and subsequent diapedesis of the neutrophils into the CSF (40, 57).

https://www.fichier-pdf.fr/2011/09/27/pathophysiologie-nitrite/

27/09/2011 www.fichier-pdf.fr

Dissertation D Frein 62%

i Abbreviations 3-NT ADH Ang II Arg ALR2 ALS BAEC BCA BH4 BKCa BSA CaM CBS cGMP CSE Cys CysNO DAF-2 DA DAN deoxyHb DMSO DTNB DTPA DTT EDRF EDTA EPR ESI EtOH FAD FMN FPLC Grx GSH GSNO GSSG Hb HbNO HbSNO 3-Nitrotyrosine Alcohol dehydrogenase Angiotensin II L-Arginine Aldose reductase (aldehyde reductase 2) Amyotrophic lateral sclerosis Bovine aortic endothelial cells Bicinchoninic acid Tetrahydrobiopterin Big-conductance Ca2+ -activated K+ channel Bovine serum albumin Calmodulin Cystathionine β-synthase Cyclic guanosine monophosphate Cystathionine γ-lyase L-Cysteine L-S -Nitrosocysteine (SNOC) DAF-2 diacetate, 4,5-diaminofluorescein diacetate 2,3-Diaminonaphthalene Deoxyhemoglobin (unliganded ferrous Hb) Dimethyl sulphoxide 5,5’-Dithio-bis(2-nitrobenzoic acid), Ellman’s reagent Diethylenetriaminepentaacetic acid Dithiothreitol Endothelium derived relaxing factor (• NO) Ethylenediamine tetraacetic acid Electron paramagnetic resonance spectroscopy Electrospray ionisation Ethanol Flavin adenine dinucleotide Flavin mononucleotide Fast protein liquid chromatography Glutaredoxin Glutathione, reduced S -Nitrosoglutathione Glutathione, oxidized Hemoglobin Nitrosylhemoglobin S -Nitrosohemoglobin ii Abbreviations HO HPLC HUVEC IC50 IRP-1 LC/MS LMW L-NAME Mb metHb mtNOS NAT NOHA NOS NOS-1 NOS-2 NOS-3 NOX oxyHb PG PGHS-2 PGI2 PN ppm PTP RLU RNS ROS SD sGC SIN-1 SMC SNAP SNP SOD SOD1 SOD2 SOD3 TCA TLC Trx Tx VEGF VSMC w/o XO YC-1 Heme oxygenase High pressure liquid chromatography Human vascular endothelial cells Half-maximal inhibition concentration Iron regulatory protein-1 Liquid chromatography/mass spectroscopy Low-molecular-weight N ω -Nitro-L-arginine methyl ester Myoglobin Methemoglobin (ferric Hb) Mitochondrial NOS 2,3-Naphthotriazole N ω -Hydroxy-L-arginine Nitric oxide synthase Neuronal NOS (nNOS) Inducible NOS (iNOS) Endothelial NOS (eNOS) NADPH oxidase Oxyhemoglobin (oxygenated ferrous Hb) Prostaglandin Prostaglandin H synthase-2 Prostacyclin Peroxynitrite parts per million Permeability transition pore Relative light unit Reactive nitrogen species Reactive oxygen species Standard deviation Soluble guanylate cyclase 3-Morpholino-sydnonimine Smooth muscle cells N -(Acetyloxy)-3-nitrosothiovaline Sodium nitroprusside Superoxide dismutase Cu,Zn-SOD, cytosolic Mn-SOD, mitochondrial EC-SOD, extracellular Cu,Zn-SOD Tricarboxylic acid Thin-layer chromatography Thioredoxin Thromboxane Vascular endothelial growth factor Vascular smooth muscle cells without Xanthine oxidase 1-Benzyl-3-(5-hydroxymethyl-2-furyl)indazole iii Publications Results from this work were published in the following articles:

https://www.fichier-pdf.fr/2011/09/26/dissertation-d-frein/

26/09/2011 www.fichier-pdf.fr

hyperthyroidism and pulmonary (4) 57%

The incidence of PE thus reflects the presence of risk factors for VTE.[12] Previous intervention studies, performed in healthy volunteers, showed a dose dependent increase in factor VIII and IX levels, von Willebrand factor, and endothelium‑associated proteins concentrations during administration of oral thyroid hormones.[25‑27] In line with these findings, van Zaane et al., demonstrate a gradual relation between plasma FT4 levels and the risk of venous thrombosis.

https://www.fichier-pdf.fr/2013/08/03/hyperthyroidism-and-pulmonary-4/

03/08/2013 www.fichier-pdf.fr

hyperthyroidism-and-pulmonary-4 57%

The incidence of PE thus reflects the presence of risk factors for VTE.[12] Previous intervention studies, performed in healthy volunteers, showed a dose dependent increase in factor VIII and IX levels, von Willebrand factor, and endothelium‑associated proteins concentrations during administration of oral thyroid hormones.[25‑27] In line with these findings, van Zaane et al., demonstrate a gradual relation between plasma FT4 levels and the risk of venous thrombosis.

https://www.fichier-pdf.fr/2013/09/05/hyperthyroidism-and-pulmonary-4/

05/09/2013 www.fichier-pdf.fr

A Growing Need for Capillaroscopy 34%

Microvascular alterations were found in patients with antiphospholipid syndrome and anticardiolipin antibody– positive SLE patients, suggesting direct damage of the vascular endothelium is triggered by anticardiolipin antibodies (44).

https://www.fichier-pdf.fr/2012/04/03/a-growing-need-for-capillaroscopy/

03/04/2012 www.fichier-pdf.fr

SurgNeurolInt42335-3043284 082712 32%

it can also seal the endothelium and wounds simply go dry during experimental laminectomy procedures.[20] Originally, this “fusogenic” potential was exploited to induce the formation of hybridomas during the production of monoclonal antibodies as well as facilitating vesicular fusion in model membrane studies.

https://www.fichier-pdf.fr/2013/07/04/surgneurolint42335-3043284-082712/

04/07/2013 www.fichier-pdf.fr

histone modification 31%

Moreover, CD44 is involved in leukocyte binding to vascular endothelium at sites of inflammation [13].

https://www.fichier-pdf.fr/2010/10/15/histone-modification/

15/10/2010 www.fichier-pdf.fr

AqualacVol5N2- Characerization of hardness 30%

Thereby, magnesium’s role in keeping the endothelium normal is important in preventing angina and also in protecting against developing high blood pressure (Seeling et Rosanoff, 2003).

https://www.fichier-pdf.fr/2014/07/30/aqualacvol5n2-characerization-of-hardness/

30/07/2014 www.fichier-pdf.fr

OXIMED2012-741545 30%

Elevated levels of glucose are able to induce endothelium-derived vasoconstrictor prostanoids [51], suggesting a role for COX2 in diabetic vasculopathies.

https://www.fichier-pdf.fr/2016/03/01/oximed2012-741545/

01/03/2016 www.fichier-pdf.fr

wpa010003 (1) 27%

It has been demonstrated in a variety of settings that catecholamines may lead to injury of the lining intimal endothelium of the coronary arteries, leading to the development of atherosclerosis (85,86).

https://www.fichier-pdf.fr/2013/11/06/wpa010003-1/

06/11/2013 www.fichier-pdf.fr

Peds0415 Septic Shock 27%

There is further damage to the endothelium and activation of multiple inflammatory cascades, including the complement system, cytokines, and interleukins.

https://www.fichier-pdf.fr/2017/09/16/peds0415-septic-shock/

16/09/2017 www.fichier-pdf.fr

Kipnis2016Science 26%

However, depending on the experi- N E UR OI MM U NO LO GY C Meningeal/parenchymal barriers A Activated T cell Cranium BMB Dura Arachnoid Meningeal vessel CSF BBB Ventricle Subrachnoid space Pia Pathology C’ Astrocyte Macrophage Choroid plexus Cytokines Intraparenchymal vessel Choroid plexus barrier B Ventricle Epithelium Fenestrated endothelium Choroid plexus capillary Although the role of meningeal immunity in CNS function is not yet fully understood, its composition and maintenance represent an interesting aspect of tissue immunology.

https://www.fichier-pdf.fr/2016/11/23/kipnis2016science/

23/11/2016 www.fichier-pdf.fr