Interchim B Efficiency of HALO 2 C18 SP 1 6 0914

Application Note: 111

Comparable Efficiency of HALO 2 C18 (2 µm Fused-Core®) and
1.6 µm Superficially Porous (SP) C18 Columns

2
1

G0095

3

4

PEAK IDENTITIES:

Aborbance @ 254 nm

Blue = HALO 2 µm C18
N (peak 4) = 15500
Pressure = 190 bar
Plates/Bar = 82

1.
2.
3.
4.

Uracil
Pyrene
Decanophenone
Dodecanophenone

STRUCTUR
Black = SP 1.6 µm C18
N (peak 4) = 15800
Pressure = 280 bar
Plates/Bar = 56

0.0

0.5

1.0
1.5
Time, min

2.0

TEST CONDITIONS:
Column 1: 2.1 x 50 mm, HALO 2 µm C18
Part Number: 91812-402
Column 2: 2.1 x 50 mm, Superficially Porous 1.6 µm C18
Mobile Phase: 15/85: A/B
A= Water
B= Acetonitrile
Flow Rate: 0.5 mL/min
Pressure: See chart
Temperature: 25°C
Detection: UV 254 nm, PDA
Injection Volume: 0.2 μL
Sample Solvent: 20/80: Water/Acetonitrile
Response Time: 0.16 sec.
Flow Cell: 1 μL
LC System: Shimadzu Nexera
ECV: ~ 7 μL

2.5

STRUCTURES:

Uracil

Decanophenone

Pyrene

Dodecanophenone

With a HALO 2 C18 column, one can
achieve the same performance at only
68% of the back pressure of a
competitor’s superficially porous 1.6 µm
C18 column.

FOR MORE INFORMATION OR TO
PLACE AN ORDER, CONTACT:
www.advanced-materials-tech.com
® HALO and Fused-Core are registered trademarks of Advanced Materials Technology, Inc.
Comparative results presented here may not be representative for all applications.