Interchim B HALO 2 to 1 7 um Totally Porous 0914

Application Note: 113

Higher Efficiency of HALO 2 C18 (2 µm Fused-Core®) compared to a
1.7 µm Totally Porous C18 Column
HALO 2 µm C18
3 N (peak 4) = 15500

2

Pressure = 190 bar

Aborbance @ 254 nm

1

0.0

4

PEAK IDENTITIES:
1.
2.
3.
4.

Totally Porous 1.7 µm C18
N (peak 4) = 9600

Uracil
Pyrene
Decanophenone
Dodecanophenone

STRUCTUR
Pressure
= 280 bar

0.5

1.0

1.5

2.0

Time, min

TEST CONDITIONS:
Column 1: 2.1 x 50 mm, HALO 2 µm C18
Halo Part Number: 91812-402
Column 2: 2.1 x 50 mm, Totally Porous 1.7 µm C18
Mobile Phase: 15/85: A/B
A= Water
B= Acetonitrile
Flow Rate: 0.5 mL/min
Pressure: See chart
Temperature: 25°C
Detection: UV 254 nm, PDA
Injection Volume: 0.2 μL
Sample Solvent: 20/80: Water/Acetonitrile
Response Time: 0.16 sec.
Flow Cell: 1 μL
LC System: Shimadzu Nexera
ECV: ~ 7 μL

STRUCTURES:

Uracil

Decanophenone

Pyrene

Dodecanophenone

With a HALO 2 C18 column, one can
achieve more separation efficiency at less
pressure than with a competitor’s totally
porous 1.7 µm C18 column.

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www.advanced-materials-tech.com
® HALO and Fused-Core are registered trademarks of Advanced Materials Technology, Inc.
Comparative results presented here may not be representative for all applications.